Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1.0 mL per vial
Storage Conditions
-70°C or colder
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Images
Comments
Requires cells that are rapidly dividing to replicate
Effect on Host
CPE, rounding and degeneration
Recommended Host
CRFK (ATCC CCL-94)
Growth Conditions
Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 60-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
FW Scott
Source
Cat
References
King DA, Croghan DL. Immunofluorescence of Feline panleukopenia virus in cell culture: determination of immunological status of felines by serum neutralization. Can J Comp Med Vet Sci 29: 85-89, 1965. PubMed: 14281074
Slater EA, CJ Kucera. U.S. Patent Application Serial #24,408, Nov. 27, 1962.