Construction: pBR328 (linearized at PvuII) + pAN12 (linearized at SspI)
Features: E. coli selectable marker cassette: ampicillin resistance E. coli selectable marker cassette: tetracycline resistance
Applications
shuttle vector
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Kostichka K, et al. A small cryptic plasmid from Rhodococcus erythropolis; chacterization and utility for gene expression.. Appl. Microbiol. Biotechnol. submitted: .
A Rhodococcus-E. coli shuttle vector.
Shipped
frozen
Shipping Information
Freeze dried Escherichia coli DH10B containing the plasmid.