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Size (kb): 7.5000000000000000

Vector: pIIIEx423 tRNA (plasmid)

Promoters: Promoter for in vitro transcription T7

Construction: pRS423

Marker(s):ampR,HIS3

Construct size (kb): 7.5

Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: SUP4 tRNA intragenic promoter
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: SalI
terminator: RPR1

YE-type (episomal) shuttle vector

vector permitting RNA synthesis in vitro

vector permitting expression of small structured RNAs

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): EcoRI--7.5; BamHI--7.5; HindIII--5.1, 2.2, 0.2.

Cloned inserts can be transcribed from the SUP4 tRNA gene intragenic promoter to produce structured pre-tRNA leaders fused to RNA corresponding to inserted sequences.

One of a series of shuttle vectors (ATCC 87167 - 87182) designed for stable expression of small structured RNAs in yeast. The vectors differ in promoter/terminator cassettes, selectable markers, replicons, and copy number.

Constructed by insertion of a promoter/terminator cassette into the multiple cloning site of the shuttle vector pRS423.

Temperature: 37.0°C