ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day to day test variation, and lot to lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen Specification range: 1 x 105 to 1 x 106 copies/µL 100 µL per vial with Biomatrica DNAstable
Storage Conditions
-20°C or colder
Intended Use
The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.
Comments
Manufactured under ISO 13485 guidance
Preparation includes fragments from the highly conserved precore, core, P, S, and X regions.
The following primers and probe can be used with this nucleic acid preparation RefSun S, et al. Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection. Virol. J. 8: 227, 2011. PubMed: 21569595:
Forward: GTCCTCCAATTTGTCCTGG
Reverse: TGAGGCATAGCAGCAGGAT
Probe: CTGGATGTGTCTGCGGCGTTTTATCAT
Special Collection
DNA
References
Sun S, et al. Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection. Virol. J. 8: 227, 2011. PubMed: 21569595
Welzel TM, et al. Real-time PCR assay for detection and quantification of hepatitis B virus genotypes A to G. J. Clin. Microbiol. 44(9): 3325-3333, 2006. PubMed: 16954268