Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx
商品貨號(hào)
B223514
Strain Designations
PO1f
Synonym
Endomycopsis lipolytica Wickerham et al., Saccharomycopsis lipolytica (Wickerham et al.) Yarrow
Alternate State
Candida lipolytica (Harrison) Diddens et Lodder
Application
transformation host
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain
no
Genotype
MATA ura3-302 leu2-270 xpr2-322 axp2-deltaNU49 XPR2::SUC2
Phenotype
Leu-, Suc-, Ura-
Preceptrol®
no
Comments
Unable to produce extracellular protease
Morphology
On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 μm. Pseudohyphae present.
Medium
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Nucleotide (GenBank) :
KU729094
ITS including 5.8S rRNA gene
Nucleotide (GenBank) :
KU729198
D1/D2 region of 26S rRNA gene
References
Madzak C, et al. Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. J. Mol. Microbiol. Biotechnol. 2: 207-216, 2000. PubMed: 10939246
Swennen D, et al. Secretion of active anti-Ras single-chain Fv antibody by the yeasts Yarrowia lipolytica and Kluyveromyces lactis. Microbiology 148: 41-50, 2002. PubMed: 11782497
