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HuCK
HuCK
規(guī)格:
貨期:
編號(hào):B224317
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HuCK
商品貨號(hào) B224317
Designations HuCK
Species Homo sapiens, human
Vector
Construct size (kb): 6.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.5
Gene product: immunoglobulin kappa constant region [IGKC]
Alleles: A1, A2
Insert Size (kb) 2.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 2.7; HindIII--6.6; PstI--6.6; BamHI--6.6; PvuII--4.4, 3.2.
The insert contains HaeIII, SacI (2), MboII, AccI, and HphI sites.
The insert includes the complete exon for the constant region plus 5' and 3' flanking sequences.
The polymorphic SacI site is located 3' to the SacI site in the coding sequence of the kappa constant region.
Enzyme(s) not detecting polymorphism: ApaI, AvaII, BamHI, BclI, BglII, BstEII, EcoRI, EcoRV, HincII, HindIII, HinfI, KpnI, MboI, MspI, PstI, PvuII, TaqI, XbaI (tested on 7 unrelated Caucasians).
References

Field LL, et al. SacI RFLP recognized by a human immunoglobulin kappa light chain constant region probe. Nucleic Acids Res. 15: 3942, 1987. PubMed: 2884643

Hieter PA, et al. Evolution of human immunoglobulin kappa J region genes. J. Biol. Chem. 257: 1516-1522, 1982. PubMed: 6276389

Weir L, Leder P. Structure and expression of a human subgroup II immunoglobulin kappa gene. Nucleic Acids Res. 14: 3957-3970, 1986. PubMed: 3086847

Hieter PA, et al. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell 22: 197-207, 1980. PubMed: 6775818

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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